Journal: International journal of molecular sciences

Article Title: A Novel Rexinoid Agonist, UAB116, Decreases Metastatic Phenotype in Hepatoblastoma by Inhibiting the Wnt/β-Catenin Pathway via Upregulation of TRIM29 .

doi: 10.3390/ijms26093933

Figure Lengend Snippet: Figure 5. UAB116 affects HLM_2 cell stemness. (a) Colony forming assay was used to evaluate the clonogenic potential of HLM_2 cells. UAB116 treatment led to a significant decrease in colony formation by HLM_2 cells. (b) Representative photographs of plates from the colony forming assay. (c) HLM_2 cells were treated with increasing concentrations of UAB116 (0–50 µM) for 72 h. Immunoblotting of whole cell lysates showing reduced protein expression of stemness markers Nanog and Nestin (upper panel), as well as Oct4 and Sox2 (lower panel). GAPDH served as a loading control. (d) Immunoblotting of whole cell lysates showing decreased total CD133 protein expression. Vinculin served as an internal loading control. (e) HLM_2 cells were treated with UAB116 (0, 25, 50 µM) for 72 h, stained for CD133, and evaluated with flow cytometry. Cell surface expression of CD133 was significantly reduced following treatment with UAB116. Results are based on a minimum of three biologic replicates, presented as mean ± SEM, and analyzed using two-tailed Student’s t tests. Scale bar represents 1 cm. * p ≤0.05, ** p ≤0.01, *** p ≤0.001, **** p ≤0.0001.

Article Snippet: We employed the following primary antibodies: rabbit monoclonal antibodies against Nanog (3580S), Nestin (73349S), Sox2 (3579S), CDK4 (12790S), CDK6 (13331S), CyclinD1 (2978S), cMyc (18583S), MMP-9 (13667S), Rb (9313S), pRb Ser780 (9307S), pRb Ser795 (9301S), β-catenin (8480S), active β-catenin (8814S), phospho β-catenin (9561S), RXRα (5388S), RXRγ (5629S), TRIM29 (50292S), and anti-vinculin (13901S) from Cell Signaling Technology (Beverly, MA, USA); and rabbit polyclonal anti-Oct4 (19857) from Abcam (Cambridge, MA, USA).

Techniques: Western Blot, Expressing, Control, Staining, Flow Cytometry, Two Tailed Test

Journal: Biomaterials Research

Article Title: Injectable Brain Extracellular Matrix Hydrogels Enhance Neuronal Migration and Functional Recovery After Intracerebral Hemorrhage

doi: 10.34133/bmr.0192

Figure Lengend Snippet: List of primary antibodies

Article Snippet: Nestin (rabbit) , 1:500 , Proteintech , 19483-1-AP.

Techniques: